Primers of AJ612027_5_A

Outer primers for tetra-primer ARMS PCR:

	Orientation	Start	Len	Tm	GC%	Any compl	3' compl	Primer Seq	Product Size	Seq Size	Included Size	Pair_any	Pair_3'
1	FORWARD	295	28	62.02	28.57	4.00	2.00	GATTTGCTTCGGTAATCTTTTCATATTT	320	657	657	4.00	1.00
	REVERSE	614	28	62.04	35.71	4.00	2.00	TTTCAACTGACTAGGAGCATCTTCTTTA

Inner primers for tetra-primer ARMS PCR:

	Orientation	Start	Len	Tm	GC%	Any compl	3' compl	Score	SNP	Pos	Primer Seq	Product Size	Seq Size	Included Size	Pair_any	Pair 3'
1	FORWARD	446	28	60.86	35.71	4.00	0.00	87.55	C	473	AGTTTAGGGGAGTTCTTTCTTAATGTTC	169	657	657	4.00	0.00
2	REVERSE	502	28	63.82	25	6.00	3.00	96.39	T	473	ATTTCAATTCTTAACATTTTGATGCGAA	206	657	657	4.00	3.00

Primer view:

1	GCTTATCACTATGTTTCTCTCTCTTAGCCTCTATGTTGTTTAAATTCATAGATTTCTTAA
61	GTTGAACAAAGAAGAAAAGGCTAACTGTATGTATGTATATACTGGCAATGTCGATCTCTG
121	TAATTATGCAACCTTCTTAAAACCGCCCCTTTGATGGTGACAGACTCTTTCAATCACATA
181	TCCATCTTTTGCTTTCCTGTTAGTAGTGATGGTCCAATAATGCCAAACTACAGTTTTATT
241	TACATTGATCTATCTTTTTGGAAAACTCTGATCTTACTTTGGTGTGCTCTGGCTGATTTG
	>>>>>>
301	CTTCGGTAATCTTTTCATATTTGCTGTCTTCCTATAGGTAGAAACAGTAACCATGATCAA
	>>>>>>>>>>>>>>>>>>>>>>
361	GTGGTTTGGCTTATATAAATACCTCTCAGACACTAGGTACTCAGAAATACATAGTAGTGT
421	GCTCTTAAGGGTCAGTACAGAAGTTAGTTTAGGGGAGTTCTTTCTTAATGGTYTAGCATC
	AGTTTAGGGGAGTTCTTTCTTAATGTTC *
	* AAGCGTAG
481	AAAATGTTAAGAATTGAAATAAAGTTATTTATCTTGTTAGCATAGCTACTTGAGCTCACT
	TTTTACAATTCTTAACTTTA
541	TCTGTTGTTCTGCTTGTGTAAACAGTGCTCTTGGCCATTTGAATGATAAAGAAGATGCTC
	<<<<<<<<<<<<<<
601	CTAGTCAGTTGAAAATAGTCGGTGTTCAAGCAACAGGTGGAATGATTGCTGGGGCAG
	<<<<<<<<<<<<<<

Outer forward primer:	>>>>>>>>>>
Outer reverse primer:	<<<<<<<<<<
Inner forward allele-specific primer:	Blue
Inner reverse allele-specific primer:	Brown
Target:	Red
Mismatch in allele-specific primers:	*

BatchPrimer3 v1.0