GSP: A web tool for Genome Specific Primers design in polyploid species

In a polyploid species, genes from the homeologous genomes exhibit a very high sequence similarity, especially in the coding regions. This makes it difficult to design genome-specific primers to amplify specific sequences from the individual genomes in the polyploid genome background. Development of genome-specific primers for important genes in polyploid species is very useful and critical not only for the study of sequence diversity and association mapping of genes in natural populations, but also for the development of gene-based functional markers for marker-assisted breeding. GSP is a powerful web-based platform for designing genome-specific primers that can distinguish the sequences among different genomes in a polypoid species.
Source code and command-line application:

If you used GSP in your research, please cite us:
GSP: a web-based platform for designing genome-specific primers in polyploids. Bioinformatics. [Link]



BLAST polyploid genome


Polyploid genome:        bread wheat

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